FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n02 gfp18.3420000000e3e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n02 gfp18.3420000000e3e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15968249
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG9200.08.8042941
TAGACCG31500.07.9999515
AGACCGT35250.07.5318696
CTATACT58400.07.35868744
ACACATC87200.07.30213445
CCTACAC41700.07.06830173
TATACTG72100.06.9278365
TACACTG58850.06.92009355
CACATCT65750.06.809844545
TAGACTG26200.06.6984325
CTAGACT15950.06.63005354
TACACCG30800.06.5746355
GTACCGT21300.06.54925546
GTGTAGG41650.06.53657441
GTATAAG80200.06.48063331
TCCTACA47650.06.46900562
ATCCTAC28600.06.45100931
TAGACAG28000.06.4285325
ATACACA202850.06.422193545
TATACAC245150.06.4154245
Produced by FastQC (version 0.11.2)