FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n02 dnfgfr21.3420000000e434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n02 dnfgfr21.3420000000e434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71121691
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT225700.017.79461145
CTGACGC307250.017.09191745
CCGTGCG58550.014.026459
TAGACCG150100.013.9107045
ACCGTGC80600.013.3715668
CCTTTGG331300.013.0735781
GCTAGAC60800.012.9153213
TACACTG272450.012.4040915
CTAGACT66250.012.3622444
AGACCGT165200.012.3395686
TCTGACG424850.012.28137444
CTGAGCG104500.011.9928049
CTTTGGG335200.011.8205862
CCGTCCG44250.011.7457459
TATACCG133400.011.6041795
GTGCTAG86650.011.5032451
AGTACCG72950.011.4735955
ACGGTAC103400.011.2935038
GTCTAGC62100.011.1232571
ATACACA2215350.011.10545
Produced by FastQC (version 0.11.2)