FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n01 lacz21.3410000000e400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n01 lacz21.3410000000e400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16311321
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC41850.024.9456345
CTCCGAG66200.019.30466545
TCTCCGA101200.012.98387345
TACACTG58400.011.7505695
ACCGTGC21600.011.1455618
CTGAGCG30350.010.9717259
TATACAC668450.010.851706545
GTGCTAG20350.010.7297181
GTCTAGC17250.010.70054151
CTATACT53250.010.6476294
TAGACCG32800.010.6323625
CCGTGCG16850.010.5487049
ATACACA506500.010.47012945
ATCTCCG127350.010.42377444
GTACTAG20400.010.3723851
CGGGACT51850.010.32761810
CTAGACT16800.010.1783234
TATACTG56600.010.0969285
CCTTTGG52500.010.0760321
GTCCTAC22650.09.9383151
Produced by FastQC (version 0.11.2)