FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n01 lacz18.3410000000e3f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n01 lacz18.3410000000e3f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21200739
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG64600.08.6770441
TAGACCG42650.08.6516375
TCCCGAG21750.08.37921421
TATACTG81750.07.9814855
GTATATA218600.07.89860151
TCTATAC66400.07.79351233
AGACCGT50200.07.7090036
CTAGACT22900.07.66359574
TATACCG45250.07.6572995
GTATAGC45600.07.6025781
GTATAGA70450.07.57307621
GTCTTAG24950.07.48880531
CCTAGAC15250.07.3768963
CTCCGAG21450.07.237611312
TAGGACG19150.07.1669524
TATACAG111500.07.1635285
CTAGGAC15450.07.13577373
CTACACT58800.07.11719854
TAGACAG40100.07.06967835
GTATTAG66200.07.0391031
Produced by FastQC (version 0.11.2)