FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n01 lacz15.3410000000e3d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n01 lacz15.3410000000e3d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8875609
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT341700.3849876667617963No Hit
CTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG114900.1294559055046251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT59500.028.09608745
TCTCGTA59050.027.89107144
ACCGAGG76950.025.23384733
CGAGGCT77250.025.10672835
CCGAGGC77550.025.03861834
GCTGATC66650.024.8794839
TGATCTC66800.024.58784141
GGCTGAT77050.024.50025738
ATCTCGT68700.023.77682543
CTGATCT71150.023.30593740
GATCTCG73000.022.53037842
AGGCTGA85950.022.4606737
CTCCGAG89350.021.75695218
TCCGAGC89650.021.7343419
CCGAGCC91250.021.27927220
CGAGCCC91800.021.20080221
AGACCGA92000.021.13013831
GCCCACG92300.021.01306324
CCCACGA92650.021.00653625
GACCGAG93150.020.91781632
Produced by FastQC (version 0.11.2)