FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n01 gfp21.3410000000e41d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n01 gfp21.3410000000e41d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42849133
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC108750.025.8614945
CTCCGAG173250.020.2073445
TCTCCGA275750.013.27531445
TAGACCG79250.010.9871365
ATCTCCG342450.010.79476544
CCGTGCG42100.010.4748259
TATACAC1607900.010.05823745
ATACACA1245750.09.640964545
CTCGGGG43600.09.54681730
TACACTG159750.09.5349985
CCGTCCG26250.09.34264859
CGGGACT100650.09.29934510
AGACCGT94850.09.1800796
ACCGTGC60650.09.0146388
TCGGGAC103000.08.9779559
CATCTCC413550.08.91710243
TATACCG86100.08.754165
GTCTAGT55650.08.615971
TAGACAG75800.08.6079875
GTGCTAG52450.08.5407761
Produced by FastQC (version 0.11.2)