FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n01 gfp18.3410000000e3e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n01 gfp18.3410000000e3e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15968249
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGGG27000.025.66732628
CTCCGAG32300.021.7330112
TCCGAGC32800.021.40171413
CGAGCCC33600.021.2269615
CCGAGCC34200.021.11770414
TGCCGTC34100.020.65179345
TATGCCG33800.020.36913143
CGAGACG35350.020.36827323
ATGCCGT35650.019.75388744
ACGAGGG36600.019.3652527
CCACGAG36950.019.2424120
GAGGGGT35750.019.13311829
GCCCACG37000.018.97316218
GTATGCC36800.018.76974542
CCCACGA39000.018.63480219
AGCCCAC38200.018.55321117
GAGACGA39500.018.11438824
GAGCCCA39850.018.01068116
TATCTCG39600.017.2160836
AGACGAG42650.016.8292725
Produced by FastQC (version 0.11.2)