FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n01 dnfgfr21.3410000000e437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n01 dnfgfr21.3410000000e437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71121691
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC167150.024.88879645
CTCCGAG268350.019.52725445
TAGACCG147050.014.2295525
CCTTTGG329050.014.0241811
CCGTGCG58500.013.4612539
TCTCCGA400950.013.17592844
AGACCGT166400.012.6830246
ACCGTGC78850.012.2698738
CTTTGGG334850.012.2694462
TACACTG267900.012.2197865
GCTAGAC60900.011.7485193
CTGAGCG103800.011.4881959
CTAGACT65050.011.3794674
TATACCG135350.011.2705415
CTCGGGG89800.011.19965230
ATACACA2239950.011.12947745
GTCTAGC64000.011.0442251
TATACAC2980600.011.02707745
GGGAAAC370800.010.9645746
CGGGACT285900.010.71855510
Produced by FastQC (version 0.11.2)