FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n02 hu_l_44.3420000000e9c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n02 hu_l_44.3420000000e9c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9738257
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16350.030.1372218
CACACGT16100.025.85369719
CGGGGCG6500.023.5382731
AGCACAC24250.022.17496517
CGGGGAG9550.021.910821
AGAGCAC27700.021.52498815
ACACGTC15750.021.28543520
TGCGCGG12650.020.27641316
CGGGGAC8450.020.2365251
GAGCACA30400.019.53921716
GTGCGCG13600.018.86004415
GCGCGGT14050.018.2559917
CTCGGTG15650.017.39602744
GCGGGAC6600.017.3862251
CGCGGTC14750.017.23706218
CGGGGAT15600.016.8748651
GGCGGTC9100.016.8130531
GGCGGAT18050.016.5788151
GCGGGAA18700.016.4838261
CGGCGTA5600.016.4730831
Produced by FastQC (version 0.11.2)