FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n02 hu_l_43.3420000000e9b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n02 hu_l_43.3420000000e9b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8485814
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG23350.032.665518
CACACGT20850.028.70464119
ACACGTC18300.028.03240220
AGCACAC31150.026.72515317
AGAGCAC36450.026.41938815
GAGCACA37900.024.99306116
CGGGGAG10650.023.451211
CGGGGAC8050.021.8017121
CACGTCT14250.021.78917521
CGGGGCG5350.020.6079241
CTCGGTG24050.020.39485244
GCGGGAC6200.019.5971981
CGTCTGA14700.019.13351823
CGGGGTG7650.019.118061
ACGTCTG15150.018.26772122
ACACGAG10100.016.93046220
TCTCGGT29800.016.76151543
TGCGCGG8400.016.60691516
GCGCGGT8700.016.03426417
AAGAGCA69850.015.91245114
Produced by FastQC (version 0.11.2)