FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n02 hu_l_42.3420000000e9a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n02 hu_l_42.3420000000e9a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6622212
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG184390.27844170497712845Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG62350.037.16880818
AGAGCAC88800.035.8019815
CACACGT46800.035.38423519
AGCACAC78500.035.3690517
ACACGTC37900.034.7291820
GAGCACA91600.034.63390716
GTCGCCG25250.033.86102345
GGTCGCC26650.031.99778244
CACACGA21700.029.96511519
CACGTCT21350.029.7186521
ACACGAG21350.029.5078820
ACGTCAG24050.029.00264222
AAGAGCA122700.028.84443314
ACGTCTG20750.027.21739622
CGTCTGA21550.026.41662623
GTGGTCG33750.025.39972742
AGATAGA56150.025.2045387
CACGAGA25550.024.56920821
TGGTCGC34950.024.46325543
CACGTCA28200.024.2550621
Produced by FastQC (version 0.11.2)