FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n02 hu_l_41.3420000000ea14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n02 hu_l_41.3420000000ea14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12189782
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG248840.20413818721286403Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG61500.036.47519318
CACACGT50250.033.53692619
AGAGCAC82500.033.1087115
AGCACAC74650.032.8830817
GTCGCCG35500.032.70385445
GGTCGCC35700.032.4576144
ACACGTC41250.032.34508520
GAGCACA85900.031.69346616
GTGGTCG45000.026.199742
TGGTCGC45250.025.90577943
CACGTCT30200.025.03282521
ACACGAG22450.024.75472620
CACACGA23050.024.11035219
CGTCTGA28100.023.9423823
AAGAGCA127450.023.62075614
ACGTCTG29650.023.44887522
ACGTCAG26250.022.45743822
CTCGGTG83900.020.86388444
CGGGGAC11350.020.8154221
CGGGGCG8800.020.7106691
Produced by FastQC (version 0.11.2)