FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n02 hu_h_44.3420000000ea07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n02 hu_h_44.3420000000ea07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12126676
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG274380.22626150809999376Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG155950.039.73327618
CACACGT126850.038.25916319
AGCACAC176100.037.86993817
ACACGTC101650.037.7392120
AGAGCAC195900.037.4994215
GAGCACA197500.037.1614516
GTCGCCG35550.035.18932745
CACGTCT59000.035.12233721
GGTCGCC36900.034.08483544
CGTCTGA57000.032.84403223
ACGTCTG57450.032.58609822
ACGTCAG53600.032.4079522
ACACGAG41700.032.10381720
AAGAGCA252700.030.96707214
CACACGA43600.030.9112219
CACGAGA45550.029.24213621
CACGTAG34050.028.67796921
CACGTCA63550.027.43859921
ACACGTA37500.027.41957720
GTGGTCG46250.027.34011742
Produced by FastQC (version 0.11.2)