FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n02 hu_h_43.3420000000e9f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n02 hu_h_43.3420000000e9f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10923684
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG517800.47401590891863954Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG175000.1602023639643915Illumina Single End PCR Primer 1 (100% over 41bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG124050.039.12260418
CACACGT92350.037.73887319
AGAGCAC161850.037.6869715
GGTCGCC65800.037.40828744
AGCACAC140450.037.3417717
GTCGCCG66800.036.9830145
GAGCACA166250.036.6624716
ACACGTC74750.035.03613320
CTCGGTG381550.033.02272844
ACACGAG41650.032.79050420
TCGGTGG388300.032.47185545
ACGTCAG49600.032.20838522
GTGGTCG77150.032.1380942
CACACGA42800.031.9620219
TCTCGGT396400.031.80818443
TGGTCGC79000.031.10068543
AAGAGCA216300.030.87320914
CACGAGA45850.029.7377221
GTGACTG56300.029.0548271
CACGTCA55300.028.72459821
Produced by FastQC (version 0.11.2)