FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n02 hu_h_42.3420000000e9ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n02 hu_h_42.3420000000e9ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9581474
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG803030.8381069551511595Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG314990.32874900041475874Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG169100.17648641534694975Illumina Single End PCR Primer 1 (100% over 42bp)
GTGGCTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG108870.1136255235885418Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG255150.039.2763418
GTCGCCG100900.039.09033645
AGCACAC308250.038.7950717
AGAGCAC361100.038.7622615
CACACGT193700.038.3667819
GAGCACA364350.038.30534716
ACACGTC148350.038.2350520
ACGTCAG96600.036.61572622
CACACGA69850.036.4955519
AAGAGCA418800.036.46796814
GGTCGCC109050.036.16886544
GTGACTG86050.035.7443661
TGACTGG84550.035.100222
CACGTCA101350.034.96515721
ACACGAG72800.034.83123820
AGATAGA164700.034.1252027
CACGTCT62100.034.05766721
GACTGGA87050.033.911243
CGTCTGA54800.033.34120623
ACGTCTG55900.032.80484822
Produced by FastQC (version 0.11.2)