FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n02 hu_h_41.3420000000e9df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n02 hu_h_41.3420000000e9df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11811476
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG131740.11153559470467535Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG48200.035.33661718
CACACGT40800.031.81938219
AGAGCAC77350.031.21156315
AGCACAC65950.031.01167517
GAGCACA80150.030.34577816
ACACGTC35400.029.74532320
GGTCGCC25700.027.31477244
GTCGCCG26050.027.29326245
ACACGAG18650.022.43934820
CGTCTGA21200.022.289223
CACGTCT24450.021.7174721
CACACGA19400.021.68782419
AAGAGCA125250.021.41285714
ACGTCAG26050.020.90277722
ACGTCTG23400.020.57757822
GTGGTCG35900.019.99273942
CACGAGA21550.019.10644521
CGGGGAC11900.017.7732221
TGGTCGC40850.017.51504143
CGGGGCG10000.017.3251111
Produced by FastQC (version 0.11.2)