FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n01 hu_l_44.3410000000e9c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n01 hu_l_44.3410000000e9c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9738257
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG14200.011.88354440
TGCCCCG28750.010.8778565
CTACGGC17900.010.18134242
GTGCCCC30950.09.9592424
TACGGCC17700.09.78777143
CCCCGGG31500.09.7853517
CGGCCTT17650.09.68802545
CCCGGGT33200.09.4875988
GACAGGT29000.09.3185151
CCTACGG19600.09.29826641
TCGGCCG15300.09.26464725
CCGGGTA38700.09.1275679
TCCCTAC19700.09.02264639
CGACCGG13000.08.8265942
GGCCCCG19450.08.799491
GCCCCGG36200.08.7013346
CGGCCGG16550.08.56489926
TATGTCC22800.08.28954835
GCGACCG14250.08.2177311
GGGCTAG17550.08.07662313
Produced by FastQC (version 0.11.2)