FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n01 hu_l_43.3410000000e9b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n01 hu_l_43.3410000000e9b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8485814
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCCG23300.014.3878325
GTGCCCC23700.014.2399324
CCCCGGG24000.013.7806947
CCCGGGT25750.012.9315178
GCCCCGG26600.012.5182926
CCGGGTA30450.011.969969
CCCTACG14600.011.86630940
CTACGGC15750.011.14267242
GGTGCCC32000.010.546453
CGGGTAA37250.010.50964410
GTAACCC32500.010.45342413
ATATCTC46250.010.36174445
GGGTAAC35400.010.35975211
TCAGACG23300.010.0425143
CCTACGG18400.010.02700641
TCCCTAC18050.09.97219139
AGACGTG23200.09.7948645
CGGCCTT19050.09.68464945
CAGGTGC37400.09.6346471
CAGACGT23400.09.6149964
Produced by FastQC (version 0.11.2)