FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n01 hu_l_42.3410000000e9ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n01 hu_l_42.3410000000e9ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6622212
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC189270.28581084386908784TruSeq Adapter, Index 2 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC105960.1600069584000029TruSeq Adapter, Index 2 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC22700.021.90457745
TATGCCG23050.021.08391643
ATGCCGT24400.020.00959844
GTATGCC28500.017.44693642
CTCGTAT51450.017.22976343
CGTATGC52100.017.01480545
GACGTGT48900.016.440371
TCGTATG54550.016.41560244
TATCTCG54950.016.00961140
CAGACGT29850.015.0748884
ATCTCGT59000.014.94878141
GTATCTC97350.014.69906145
TCTCGTA61700.014.69574542
TCAGACG30600.014.5583523
CGATGTA195950.014.39861645
CTACACG9350.013.9691081
AGACGTG31450.013.9502645
ACGTGTG58100.013.8248532
TGTATCT106500.013.58406744
GTTCAGA37500.012.7908851
Produced by FastQC (version 0.11.2)