FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n01 hu_l_41.3410000000ea17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n01 hu_l_41.3410000000ea17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12189782
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC230490.18908459560638574TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT57250.019.25697743
CGTATGC61900.018.90080345
GATCTCG60700.018.12577840
TCGTATG66750.017.594944
ATCTCGT63900.017.00673541
TCTCGTA70400.015.88394642
CACGATC102150.015.63822843
ACGATCT104350.015.56726544
CGATCTC114600.014.03747545
ATCACGA197000.012.5744445
GACGTGT53150.012.4999631
TCACGAT136700.012.44308342
CATCACG229800.010.79923644
ACGTGTG62700.010.2986892
CAGACGT40700.010.1163594
TCAGACG41450.09.9875933
CCCCGGG16450.09.9844597
AGACGTG41550.09.9635565
CCCGGGT16750.09.8056338
GTCACAG34750.09.7118966
Produced by FastQC (version 0.11.2)