FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n01 hu_h_44.3410000000ea0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n01 hu_h_44.3410000000ea0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12126676
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC269520.22225381464797112TruSeq Adapter, Index 12 (100% over 50bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA121520.10020882886621198TruSeq Adapter, Index 12 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCTC154100.019.6813145
GTAATCT153750.019.65294644
CTCGTAT63200.019.50878543
CGTATGC66150.019.04692845
TGTAATC160650.018.85085743
TCGTATG70500.017.90360544
ATCTCGT74150.016.4764141
GACGTGT125350.015.27152251
AATCTCG80850.015.25028940
GTCACAG42950.014.9819946
TCTCGTA83250.014.91862442
GTTCAGA77750.014.8884841
AGTCACA43750.014.6051845
TCAGACG79100.014.3357643
CAGACGT80100.014.2129684
TTGTAAT216000.014.19763642
AGTTCAG56200.013.4645161
AGACGTG83950.013.4003485
ACGTGTG149650.012.824462
TATGCCG24200.012.36525343
Produced by FastQC (version 0.11.2)