FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n01 hu_h_43.3410000000e9fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n01 hu_h_43.3410000000e9fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10923684
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC498720.45654927403612183TruSeq Adapter, Index 11 (100% over 50bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA177530.1625184324262767TruSeq Adapter, Index 11 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTC411950.030.29518545
TACATCT429850.029.10689544
CTACATC438900.028.52735543
GCTACAT473000.026.6616142
CGTATGC101150.024.31163845
CTCGTAT102200.023.97391343
TCGTATG105650.023.36130344
GGCTACA580200.021.75489641
ATCTCGT112850.021.63216841
GACGTGT84500.020.9462181
TCTCGTA117750.020.94216242
CGGCTAC623400.020.25141140
CATCTCG123000.019.95728540
ACGGCTA666700.018.96989439
CACGGCT681150.018.54434438
TCACGGC703950.017.95330437
GTCACGG720000.017.54099836
AGTCACG738200.017.15425335
TGCCGTC29200.017.02822545
GTCACAG56000.016.9142996
Produced by FastQC (version 0.11.2)