FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n01 hu_h_42.3410000000e9ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n01 hu_h_42.3410000000e9ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9581474
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC791090.825645406959305TruSeq Adapter, Index 9 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC524730.5476506015671493TruSeq Adapter, Index 9 (100% over 51bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA313950.32766357243154864TruSeq Adapter, Index 9 (100% over 40bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT166890.1741798808826283TruSeq Adapter, Index 9 (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC157630.16451539710904606TruSeq Adapter, Index 9 (100% over 51bp)
CTCCAGCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA116070.12114002501076555TruSeq Adapter, Index 9 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC74000.035.1776845
ATGCCGT76900.033.7925744
TATGCCG77800.033.48858643
GTATGCC99300.026.21567542
GACGTGT175700.022.9782261
CTACACG20450.022.2415981
CGTATGC188850.021.07542845
TCGTATG199450.020.03431744
CTCGTAT194150.019.87442643
GTCACAG84550.019.611896
TCTCGTA209350.018.46405642
AGTCACA86700.018.4248875
ACGTGTG221850.018.1128852
ATCTCGT219550.017.54475241
TACACGA26200.017.2607772
ACACGAC25350.017.2182663
CGACGCT33200.017.1454163
TCACAGA133450.017.0787667
GTTCAGA78250.016.804921
ACGACGC39350.016.4669572
Produced by FastQC (version 0.11.2)