FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l01n01 hu_h_41.3410000000e9d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l01n01 hu_h_41.3410000000e9d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11811476
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC129870.10995238867691048TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT37950.017.96365443
CGTATGC43350.016.40067745
TCGTATG43750.016.3535844
ATCTCGT46400.014.838037541
GACGTGT44350.014.42001151
TCTCGTA51500.013.892899542
AATCTCG50600.013.56207140
ACGTGTG50950.012.36453152
TCAGACG31550.011.5525923
CAGACGT31650.011.4450054
AGACGTG33000.010.9086215
CGTGTGC59250.010.5565073
GAATCTC72600.09.70028239
CACTTGA150600.08.69484444
ACTTGAA158050.08.51276245
GTGTGCT75200.08.3772984
TGAATCT85800.08.23415538
GTCACAC156000.08.22104440
TTCAGAC46400.08.1946912
ACACTTG159250.08.180181543
Produced by FastQC (version 0.11.2)