FastQCFastQC Report
Fri 5 Dec 2014
QX171-ASE-3_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameQX171-ASE-3_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79012099
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCTG17762200.044.9990041
GAGGCTC11111500.044.7601781
AGGCTGT6747750.044.6540682
GAGGCTA6009950.044.636781
AGGCTAT2638500.044.633912
AGGCTCG2891150.044.487452
AGGCTAG1201100.044.3977472
GAGGCTT8011950.044.03291
AGGCTGA2882500.043.9731182
TTACCTG449450.043.8934751
AGGCTGG5745650.043.6470072
GGCTATT1041350.043.604943
AGGCTCC2020850.043.503082
AGGCTTA959950.043.43392
AGGCTGC2996900.043.379342
AGGCTCA2378100.043.149482
GGCTAGG247300.042.983613
AGGCTCT4300600.042.9485052
GGCTGTA880600.042.868523
GGCTGTG2220300.042.6950073
Produced by FastQC (version 0.11.2)