FastQCFastQC Report
Fri 5 Dec 2014
YingC8_GGCTAC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYingC8_GGCTAC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8844939
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC235501526.625565196096883TruSeq Adapter, Index 11 (100% over 51bp)
ATCTAACAGCAATACCTGATCACTACTTCGCACTAGTTTCTCGGTACTATG1012431.144643281316016No Hit
ATCCCCGAGTCCTTCAATGCTATCATTCCCTTTGATATTGGACCATATGCA716210.8097398975843699No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA659400.7455110770125153No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC482170.5451366029771375No Hit
ATCCCCGAGTCCTTCAATGCTATCATTCTCTTTGATATTGGACCATATGCA209200.23651943783897209No Hit
CCTTAACAGCAATACCTGATCACTACTTCGCACTAGTTTCTCGGTACTATG166970.18877462015283542No Hit
CCTCACGAGTCCTTCAATGCTATCATTTCCTTTGATATTGGATCATATGCA155770.1761120116260836No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC104600.11825971891948604No Hit
ATCCCCGAGTCCTTCAATGCTATCATTTCCTTTGATATTGGATCATATGCA91050.10294022378221036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2600950.044.5183839
GCACACG2595950.044.4905911
TCGGAAG2602600.044.4900323
AGAGCAC2605150.044.482888
AGCACAC2602850.044.46254710
CACACGT2598300.044.43995712
CGGAAGA2607200.044.3934144
ATCGGAA2614300.044.3899082
ACACGTC2598200.044.38365613
GATCGGA2608900.044.380721
ACGTCTG2598500.044.33582715
CGTCTGA2601300.044.27340316
AAGAGCA2620000.044.269117
GAAGAGC2622550.044.2285356
CACGTCT2609000.044.1645714
TCTGAAC2601500.044.14373818
GGAAGAG2630150.044.115565
GTCTGAA2607550.044.0853217
CTGAACT2601500.044.068519
GAACTCC2602700.043.91438321
Produced by FastQC (version 0.11.2)