FastQCFastQC Report
Fri 5 Dec 2014
YingC6_GATCAG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYingC6_GATCAG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8864338
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC210318823.726396714565713TruSeq Adapter, Index 9 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA746400.8420256538051686No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC527120.5946524151042074No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCAGATCTCGTATGCC135180.1524986975902769TruSeq Adapter, Index 9 (98% over 51bp)
ATCTAACAGCAATACCTGATCACTACTTCGCACTAGTTTCTCGGTACTATG130590.14732064594107308No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC117620.13268898365563225No Hit
ATCCCCGAGTCCTTCAATGCTATCATTCCCTTTGATATTGGACCATATGCA90430.10201551430010904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2324850.044.42024611
CACACGT2324950.044.40285512
GAGCACA2332850.044.3740049
ACACGTC2324050.044.3697113
AGCACAC2333050.044.356710
AGAGCAC2338550.044.3120278
ACGTCTG2323300.044.3029115
CGTCTGA2323950.044.2624416
TCGGAAG2342400.044.0855453
AAGAGCA2355400.044.0668757
TCTGAAC2329550.044.06525818
CACGTCT2338250.044.06369414
GTCTGAA2332900.044.0415217
CGGAAGA2346800.044.008644
CTGAACT2330800.043.95475419
GAAGAGC2363550.043.9037486
ATCGGAA2358950.043.86332
GAACTCC2332200.043.79958321
ACTCCAG2322300.043.79686723
TGAACTC2340900.043.7497320
Produced by FastQC (version 0.11.2)