FastQCFastQC Report
Fri 5 Dec 2014
YingC5_ACTTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYingC5_ACTTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9593090
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC327472334.13626891856534TruSeq Adapter, Index 8 (100% over 51bp)
ATCTAACAGCAATACCTGATCACTACTTCGCACTAGTTTCTCGGTACTATG425060.4430897656542365No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA316070.3294767379436657No Hit
ATCCCCGAGTCCTTCAATGCTATCATTCCCTTTGATATTGGACCATATGCA282010.29397201527349376No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC220720.23008227797299935No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGATCTCGTATGCCG142490.14853399686649454TruSeq Adapter, Index 8 (97% over 39bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC131050.13660874650399402TruSeq Adapter, Index 8 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA3637650.044.4255459
GCACACG3625250.044.42048311
AGAGCAC3643200.044.407898
AGCACAC3639450.044.37698710
TCGGAAG3646700.044.3395773
CACACGT3631150.044.3359212
ACACGTC3628550.044.303213
AAGAGCA3659300.044.2801327
CGGAAGA3651050.044.262714
ATCGGAA3663450.044.2439582
GAAGAGC3663950.044.2315376
ACGTCTG3626150.044.22767615
CGTCTGA3626850.044.20053516
GATCGGA3663950.044.170511
GGAAGAG3673150.044.123835
CACGTCT3641850.044.10310414
GTCTGAA3635000.044.0574917
TCTGAAC3635150.044.0309118
CTGAACT3633150.043.98703819
GAACTCC3636700.043.8406221
Produced by FastQC (version 0.11.2)