FastQCFastQC Report
Fri 5 Dec 2014
YingC4_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYingC4_GCCAAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11689999
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC394619633.75702598434781TruSeq Adapter, Index 6 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA440580.3768862597849666No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC330690.28288283001563985No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC156120.13355005419589855TruSeq Adapter, Index 6 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG4264100.044.569343
GAGCACA4265300.044.551429
AGAGCAC4270100.044.5461278
GCACACG4257150.044.51621211
AGCACAC4265750.044.50980410
GATCGGA4273000.044.4963341
ATCGGAA4278800.044.4937172
CACACGT4258450.044.48677412
CGGAAGA4269350.044.483444
ACACGTC4261400.044.43274313
GAAGAGC4288800.044.3916746
AAGAGCA4289850.044.390927
ACGTCTG4262550.044.3743815
CGTCTGA4264500.044.33245516
GGAAGAG4300850.044.2962845
CACGTCT4273750.044.27276214
TCTGAAC4272150.044.2030418
GTCTGAA4277950.044.16309417
CTGAACT4272350.044.1346219
TGAACTC4281600.044.02876320
Produced by FastQC (version 0.11.2)