FastQCFastQC Report
Fri 5 Dec 2014
YingC1_CGATGT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYingC1_CGATGT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10033203
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC125505912.509056180763011TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1402550.044.34734713
CGTCTGA1406700.044.30431416
ACGTCTG1409350.044.2401615
CACACGT1405400.044.220612
GCACACG1407550.044.1770311
CACGTCT1409000.044.14114414
GTCACCG1387000.043.90977529
ACCGATG1373850.043.87021632
CGATGTA1365250.043.86148534
CCGATGT1367250.043.84010733
GAGCACA1427000.043.816129
AGCACAC1426050.043.8090210
AGAGCAC1428500.043.8047568
CACCGAT1377200.043.79780631
TCACCGA1379500.043.7900230
GTATGCC1407900.043.7318945
TATCTCG1387800.043.71779639
TCTGAAC1423700.043.6978618
CGGAAGA1440150.043.657984
TCGGAAG1441200.043.640233
Produced by FastQC (version 0.11.2)