FastQCFastQC Report
Fri 5 Dec 2014
Ying8i_CCGTCC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYing8i_CCGTCC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9005315
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC6203136.888298743575322TruSeq Adapter, Index 16 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG688250.044.49313711
ACGTCTG692100.044.18214815
AGCACAC696400.044.09196510
TCGGAAG698050.044.0865973
GAGCACA697250.044.083399
CACACGT694250.044.0632412
AGAGCAC698750.044.0402688
CGTCTGA693950.044.03518316
ACACGTC694550.043.99237413
CCCGTCC661200.043.75288433
GTCACCC676900.043.65635729
TCACCCG671950.043.65221830
GTATGCC689100.043.57160645
CCGTCCA664050.043.5617134
AGTCACC683250.043.5539328
CACCCGT668550.043.54780231
TCTGAAC702450.043.46390518
ATCGGAA710100.043.440112
ACCCGTC668850.043.38363632
CGGAAGA710400.043.329674
Produced by FastQC (version 0.11.2)