FastQCFastQC Report
Fri 5 Dec 2014
Ying7i_ATGTCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYing7i_ATGTCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9553531
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC118505812.40439791319042TruSeq Adapter, Index 15 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1287000.044.52746211
TCGGAAG1293000.044.4220053
GAGCACA1294450.044.3702669
CACACGT1291900.044.33593412
AGAGCAC1297150.044.3178028
AGCACAC1295950.044.29633310
ACGTCTG1290800.044.28598815
ACACGTC1293550.044.2602513
CGTCTGA1293950.044.16253316
ATCGGAA1306200.044.036842
CGGAAGA1306600.043.956194
TCTGAAC1299150.043.8264518
GATCGGA1312650.043.7625851
CTGAACT1301150.043.66571819
AAGAGCA1319550.043.6296967
GTCTGAA1308100.043.61258317
ACTCCAG1295900.043.56463623
GTATGCC1280900.043.5467945
GTCACAT1280150.043.4304129
GAAGAGC1327200.043.397546
Produced by FastQC (version 0.11.2)