FastQCFastQC Report
Fri 5 Dec 2014
Ying6i_AGTTCC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYing6i_AGTTCC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9162463
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC107429511.724958671047293TruSeq Adapter, Index 14 (98% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA163590.17854369507413018No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC113720.12411509874582849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1202950.044.4767611
TCGGAAG1206150.044.3863753
CACACGT1206150.044.3680912
ACACGTC1206200.044.34013713
GAGCACA1210450.044.318289
AGAGCAC1212050.044.298768
ACGTCTG1205150.044.28283715
AGCACAC1211150.044.27409410
CGTCTGA1208250.044.15059716
ATCGGAA1220300.043.9712752
CGGAAGA1219900.043.904534
TCTGAAC1213150.043.85543418
GTCTGAA1220750.043.64138417
GATCGGA1227400.043.621611
CTGAACT1218550.043.56508319
AAGAGCA1235200.043.5461247
ACTCCAG1212100.043.54103523
CACAGTT1180300.043.46534331
TCACAGT1182200.043.4525830
GTATGCC1194000.043.42167745
Produced by FastQC (version 0.11.2)