FastQCFastQC Report
Fri 5 Dec 2014
Ying5i_AGTCAA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYing5i_AGTCAA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8765994
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC116913113.337118414637292TruSeq Adapter, Index 13 (98% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA94230.10749494010605072No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCAAATCTCGTATGCC93420.10657091483293282TruSeq Adapter, Index 13 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1313600.044.5763311
TCGGAAG1319050.044.5462073
CACACGT1314600.044.50990312
GAGCACA1320050.044.498289
ACACGTC1315050.044.45874413
AGAGCAC1323550.044.4503028
AGCACAC1321500.044.41029410
ACGTCTG1315150.044.39396715
CGTCTGA1318300.044.26911516
ATCGGAA1335850.044.0735782
CGGAAGA1333550.044.0483444
TCTGAAC1322000.043.98354318
AAGAGCA1344250.043.841317
CTGAACT1326950.043.7414819
GATCGGA1347200.043.6408421
GTCTGAA1335100.043.62780817
GAAGAGC1354150.043.5409746
ACTCCAG1323000.043.47290823
CACAGTC1268600.043.4443531
TCACAGT1273650.043.4222430
Produced by FastQC (version 0.11.2)