FastQCFastQC Report
Fri 5 Dec 2014
Ying1i_CAGATC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYing1i_CAGATC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8558755
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC5627546.575185292720728TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG633850.044.29180511
CACACGT635650.044.15930612
TCGGAAG641050.044.1221353
ACACGTC636150.044.0892313
ACGTCTG635150.044.08522415
AGAGCAC641750.043.9674458
AGCACAC640400.043.9547310
GAGCACA641550.043.932059
CGTCTGA638550.043.8328716
AGTCACC626700.043.4497628
ATCGGAA652550.043.4376642
CGGAAGA652900.043.317884
TCTGAAC645300.043.2941818
GTATGCC633450.043.23419645
CCAGATC615900.043.12920433
ACTCCAG644850.043.00677523
GATCGGA659050.042.944521
GTCTGAA652050.042.9288117
CACCAGA623200.042.91616431
ACCAGAT620550.042.9109132
Produced by FastQC (version 0.11.2)