FastQCFastQC Report
Wed 29 Oct 2014
uncut_9_old_TCTCGCGC-GTACTGAC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_9_old_TCTCGCGC-GTACTGAC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5284784
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA756941.4323007335777582No Hit
GGGGAACACGATGATATTCACCACACCGCTGGCGTTCAGCGCGTAGGCTTC62680.11860465820362762No Hit
GGGGTGGAGCAAGCGTGACAAGTCCACGTATGACCCGACCGACGATATCGA62020.11735578975413186No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG61530.11642859954162743TruSeq Adapter, Index 8 (97% over 36bp)
ATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTTGAGGAC55160.10437512677906988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG7150.040.9076140
CGTAGGC7900.037.0239842
GCGTAGG8000.036.84241541
GGGGTGG7650.036.1984521
GACCGAC8300.035.781537
CCACGTA8450.035.14632824
CTGGCGT8350.035.02999529
ATGACCC8650.034.59543230
CGACCGA8600.034.53330636
GTAGGCT8800.034.00477243
GTCCACG8800.033.74814222
CGCTGGC8950.033.18284227
CCCGACC9500.032.68426534
GGGTGGA8750.032.666472
CAGCGCG9050.032.56757737
GCGCATC11050.031.96716938
TGACCCG9400.031.83516131
GCTGGCG9400.031.595228
CGTGACA9600.031.40422214
CACGATG9750.030.6903277
Produced by FastQC (version 0.11.2)