FastQCFastQC Report
Wed 29 Oct 2014
uncut_9_old_TCTCGCGC-GTACTGAC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_9_old_TCTCGCGC-GTACTGAC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3326486
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA307820.9253608763121204No Hit
GGGGTGGAGCAAGCGTGACAAGTCCACGTATGACCCGACCGACGATATCGA42170.12677041177987822No Hit
GGGGAACACGATGATATTCACCACACCGCTGGCGTTCAGCGCGTAGGCTTC41360.12433540979880872No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG41020.12331331020181659TruSeq Adapter, Index 8 (97% over 36bp)
ATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTTGAGGAC35830.10771126047126006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG4950.040.456640
GCGTAGG5000.040.05203241
CCACGTA6300.039.64129624
GGGGTGG5950.039.3492471
GTCCACG6600.037.83941722
GACCGAC6650.037.5571737
CGACCGA7050.036.06403436
ATGACCC6950.035.6111830
GCAAGCG7150.035.2428369
CGTGACA7250.035.0670614
GGGTGGA7100.034.8619732
CGTAGGC5800.034.52761542
TGACCCG7250.034.44795231
CAGCGCG5850.034.2314837
CTGGCGT6100.033.19541529
GTAGGCT6200.032.3000343
ACGATAT7850.031.81690242
TCCACGT8000.031.2175223
AAGCGTG8150.031.19462211
CCCGACC8050.031.02502334
Produced by FastQC (version 0.11.2)