FastQCFastQC Report
Wed 29 Oct 2014
uncut_8_TAATGCGC-GGCTCTGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_8_TAATGCGC-GGCTCTGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058248
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42460.20629195315627658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC856.4683263E-926.4780642
TGCGCGC1900.022.5063553
ATCTCGA16650.021.3636111
CTGCGCG1503.43789E-1019.5055082
TCTCGAG19650.018.3257852
ATATCTC6100.018.0841481
ATATGGA12750.017.4806211
TATCTCG6900.016.9613112
ATTCGGC1004.15087E-415.7590441
TATGGAT14200.015.5325542
CTCGAGG26000.014.1097543
TCGAGGG26950.013.9464234
TCGCGCC1709.384017E-613.2390323
CGAGGGC30100.012.4121435
TGAGGGC1651.040923E-412.2761943
CCGGCGC55300.012.0910251
TTCGGCG1450.006813341810.8651362
CGGCGCG64500.010.5378592
TGGATCC22100.010.3875484
GAGGGCG36100.010.3456626
Produced by FastQC (version 0.11.2)