FastQCFastQC Report
Wed 29 Oct 2014
uncut_8_TAATGCGC-GGCTCTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_8_TAATGCGC-GGCTCTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3308306
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38230.11555762979603458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA26900.021.7603021
GTCGCGC1900.021.317662
TCTCGAG30500.019.9935572
CTGCGCG2100.018.2158832
ATATGGA19950.017.0403251
ATATCTC9700.016.7110841
TGCGCGC2850.016.58043
TCGAGGG39400.016.5050054
CTCGAGG37550.016.2397733
TATGGAT21650.015.7981512
TATCTCG10800.015.4259461
TCGCGCC2701.8189894E-1215.0013153
CGAGGGC44400.014.6970135
TGGCGCG3750.012.6075841
GAGGGCG53200.012.3071076
CCGGCGC86900.012.2023931
AGGGCGC56050.011.6809697
ATTCGGC2402.2486347E-611.2567711
GGGCGCG58600.011.2110628
CCGAGGG2301.7347802E-510.7618133
Produced by FastQC (version 0.11.2)