FastQCFastQC Report
Wed 29 Oct 2014
uncut_7_TAATGCGC-CCTATCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_7_TAATGCGC-CCTATCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034217
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64160.3154039121686624No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT21110.10377457272257581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC504.875801E-836.005312
TGCGCGC1004.5474735E-1127.0033193
GGTCGCG500.002258741822.5110661
TCGCGCC1056.105802E-821.4312063
ATATGGA11300.019.1244471
TATCTCG7100.017.7490982
ATCTCGA14050.016.1823331
TATGGAT13800.015.8175512
CTGCGCG1056.032936E-415.00221352
ATATCTC8800.014.3252251
TCTCGAG16200.014.3076662
TCGAGGG20750.012.4714134
CTCGAGG19850.012.0165913
GGATCCG20350.011.1684485
CGAGGGC23350.011.0827335
ATGGATC21150.010.4268133
TGGATCC22750.010.1880664
GATCCGG27650.09.8511371
GAGGGCG27300.09.4773246
AGGGCGC28550.09.0623797
Produced by FastQC (version 0.11.2)