FastQCFastQC Report
Wed 29 Oct 2014
uncut_7_TAATGCGC-CCTATCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_7_TAATGCGC-CCTATCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736546
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74660.1998101990447863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGC1950.027.695713
GTCGCGC1004.5474735E-1127.0033172
TCGCGCC1301.2278178E-920.7717823
CTGCGCG1653.6379788E-1220.4570582
ATATGGA22300.020.0892771
TATGGAT25150.018.0737922
TATCTCG13450.015.565991
ATATCTC14500.014.5940531
ATCTCGA25200.013.1320621
CGTCGCG1403.5934846E-412.8640591
TCTCGAG28800.012.1889972
GGATCCG39550.011.7768925
TGGATCC40000.011.75690654
ATGGATC39850.011.5196083
TCGAGGG33850.011.034594
TATACGT6700.010.7468695
CTCGAGG33550.010.2620653
ATACGTA6650.010.1506716
TGGCGCG2251.6431884E-410.0012283
TACGTAT6850.09.8536427
Produced by FastQC (version 0.11.2)