FastQCFastQC Report
Wed 29 Oct 2014
uncut_6_TAATGCGC-ATAGAGGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_6_TAATGCGC-ATAGAGGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5007248
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62113612.404738091662326No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162710.324948953996287No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141910.2834091700670708No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104210.2081183116953664No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103970.20763900649618314No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102650.2050028279006752No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73950.1476859144983432No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT68560.13692151856668575No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT66550.1329073375235259No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64760.1293325195796174No Hit
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63170.12615712263502826No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58810.11744974484986564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCGC2850.024.4781443
ATATGGA30000.024.4625191
ATATCTC18600.023.6007881
ATAAAAA40600.021.8389552
AATAAAA41100.021.3065241
TATCTCG22150.019.7101362
GTCGCGC1555.347829E-1018.8744072
TGCGCGC2900.018.6240833
GATCCGG114550.018.3550931
TATGGAT40900.017.827212
ATCTCGA43900.017.383611
TGCGCAT2850.015.7890437
TAATGCG3000.015.75066934
TCTCGAG51100.015.7220432
TTCGGCG4450.015.6770142
ACCCGGC2850.015.0027342
ATCCGGC142400.014.8394322
TCGGCGC5250.014.5740823
ATTCGGC5300.014.0166071
CCCGGCG2957.2759576E-1213.7313163
Produced by FastQC (version 0.11.2)