FastQCFastQC Report
Wed 29 Oct 2014
uncut_6_TAATGCGC-ATAGAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_6_TAATGCGC-ATAGAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5070431
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3863987.620614500029682No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165800.3269938985462972No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141800.27966064423320225No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105940.20893687341371966No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101370.1999238329049345No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95310.18797218619087805No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76290.15046058214775038No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT68820.1357281067427996No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT64710.12762228694168207No Hit
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61600.12148868607027687No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59430.11720897099280121No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57630.11365897691931909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA31250.024.2023331
TGGGCGC3300.023.8655873
ATATCTC18950.023.4004921
TATCTCG22800.019.7384552
GCCCCCC600.00651004518.75171744
TATGGAT41250.018.546972
GATCCGG110150.017.6561991
ATCTCGA45150.017.249891
ATAAAAA33850.016.2864132
AATAAAA34700.015.6983451
TGCGCGC3350.015.4490243
TCTCGAG53550.015.0852662
ATCCGGC134500.014.6220132
TGGATCC56650.014.1796314
TCGAGGG61550.013.7453694
GCGCATC2957.2759576E-1213.72966538
ACCCGGC2804.0017767E-1113.6618322
CTCGAGG60700.013.4195483
TCGGCGC4700.013.4053513
ATGGATC60350.012.9753773
Produced by FastQC (version 0.11.2)