FastQCFastQC Report
Wed 29 Oct 2014
uncut_5_TAATGCGC-TATAGCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_5_TAATGCGC-TATAGCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709060
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27389410.110296560430555No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255470.9430208264121134No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173100.6389670217713893No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115930.42793441267450705No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103360.38153455442109074No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88780.327715148427868No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT87520.3230640886506759No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82060.3029094962828435No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT61040.2253180069839723No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57360.2117339593807446No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT55360.20435132481377302No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48290.17825371161952855No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46560.17186773271909814No Hit
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45070.16636766996670432No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44770.16526027478165858No Hit
GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43310.15987095154776934No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT41280.1523775774622932No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37780.13945796697009294No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32170.11874967700973771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCGC1700.031.7702833
ATATGGA23650.030.1722151
ATATCTC12050.028.9550081
AACCGGC4000.028.1304592
TGCGCGC1950.025.3890723
ACCGGCG4400.025.0612183
AATAAAA17400.023.4157661
CCCGGCG1750.023.1469213
TGGCGCG2200.022.503953
ATCTCGA35850.020.5323031
ACCCGGC1900.020.1354852
TATGGAT36200.020.0176982
GATCCGG103850.019.5514551
ATAAAAA21350.019.500272
TATCTCG18950.019.1198042
TCTCGAG40350.018.5723762
GAACCGG6200.018.5164431
GTTCCGG1951.8189894E-1218.4651222
TCTGGCG1102.2485328E-618.4174231
GTGGGCG3350.018.1378482
Produced by FastQC (version 0.11.2)