FastQCFastQC Report
Wed 29 Oct 2014
uncut_5_TAATGCGC-TATAGCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_5_TAATGCGC-TATAGCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4114460
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2605146.331669283453965No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398880.9694589326424367No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271190.659114440291071No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189350.4602061995984892No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156410.38014709099128435No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT136760.3323886974232341No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129220.3140630848276566No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123570.3003310276439679No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93130.22634805053397045No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT91380.22209475848592528No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT80300.195165343690302No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71830.1745794101777633No Hit
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67390.16378820063872296No Hit
GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66430.16145496614379531No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63480.15428513097709057No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT59100.14363974859398315No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58160.14135512315103319No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50550.12285937887353383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCGC2850.031.5845243
ATATCTC17700.030.2757741
ATATGGA32350.027.562111
GAGCCGG4300.025.6577871
ACCCGGC2400.025.3169732
CCCGGCG2800.022.5039753
TGCGCGC3800.021.3195553
GTCGCGC1501.4551915E-1121.003712
GATCCGG155350.020.5955961
TATCTCG27250.020.2329332
GTGGGCG4300.019.8872322
ATCTCGA51950.019.547111
TTCGGCG4200.019.2891222
AATAAAA21950.018.464161
ACCGGCG8300.018.165863
TATGGAT50450.018.0210232
AACCGGC7650.017.9443472
TCTCGAG58850.017.5519542
ATGCGCA1953.45608E-1117.30738336
TGCGCAT2005.0931703E-1116.87469937
Produced by FastQC (version 0.11.2)