FastQCFastQC Report
Wed 29 Oct 2014
uncut_4_CTGAAGCT-GGCTCTGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_4_CTGAAGCT-GGCTCTGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189179
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1432744.492504183678621No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144700.45372178858571444No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92810.29101533654899897No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT62460.195849778265817No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61200.19189891818552673No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59240.18575313583840855No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50580.15859881179450888No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37520.11764783350197652No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT36820.1154529112351486No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35690.11190967957584068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA21650.026.4091511
TATGGAT28600.019.6705462
GATCCGG74800.018.9891911
TATCTCG12850.018.7379932
TGGGCGC1751.382432E-1018.0024853
ATATCTC13700.017.745241
TGCGCGC1651.2296368E-917.7297173
CTGCGCG1803.910827E-916.2522432
ATCTCGA26800.015.53873351
ATCCGGC92200.015.37630752
ATGGATC37200.015.304533
TGGCGCG3100.015.2440393
TCGGCGC2751.8189894E-1214.7293053
TGGATCC38800.014.6734164
GTGGGCG2152.581146E-914.6531852
ACCCGGC1859.613723E-814.5966092
GGATCCG41000.013.7762925
TCTCGAG31250.013.7538982
TCGAGGG33300.013.6505334
CGAGGGC45100.013.126771
Produced by FastQC (version 0.11.2)