FastQCFastQC Report
Wed 29 Oct 2014
uncut_4_CTGAAGCT-GGCTCTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_4_CTGAAGCT-GGCTCTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5304541
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1479352.7888369606342946No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239550.4515942095649746No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147390.27785627446370953No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99150.18691532405914102No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT99140.1868964722866691No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98800.18625551202262364No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78330.1476659337725922No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59700.11254508165739505No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57950.10924602147480811No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT56650.1067952910534578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA34550.021.8939341
GATCCGG119100.018.6001281
TATCTCG23600.018.211132
TGGGCGC2650.017.8313773
TATGGAT43850.017.7550542
ATATCTC24900.016.6360841
ATCTCGA45100.016.173391
GTGGGCG3500.015.4297462
ATCCGGC144750.015.3897722
TCTCGAG51050.014.5897472
TCGAGGG55450.013.7968714
TGGATCC59500.013.576284
CGAGGGC77050.013.4697911
ATGGATC59100.013.4019083
TGCGCGC3201.8189894E-1213.3602653
CGTGGGC3900.013.2768751
ACCCGGC3900.013.2702412
CCGGCGC185400.013.1074224
TCCGGCG173200.013.01761153
CTCGAGG56850.012.9428153
Produced by FastQC (version 0.11.2)