FastQCFastQC Report
Wed 29 Oct 2014
uncut_3_CTGAAGCT-CCTATCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_3_CTGAAGCT-CCTATCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4322263
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4165449.637173860082092No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147730.34178854919286494No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88110.20385154721033866No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70080.1621372878050225No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68140.15764889827389034No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT64760.14982892063717548No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62820.14534053110604328No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62770.14522485096348833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCGC1653.6379788E-1220.456463
TGGCGCG2850.019.738693
ATATGGA25350.019.6240331
ATAAAAA30550.019.5190122
ATATCTC15600.018.6139721
AATAAAA32000.018.2893091
TGCGCGC2350.018.1931923
GATCCGG77650.018.0891061
TATCTCG17050.017.2890072
ATCTCGA35150.016.5222171
CTGCGCG2500.016.2015172
TCTCGAG39250.015.2498352
TATGGAT33350.015.1813322
GTCGCGC1654.2031206E-715.0014052
ATCCGGC96700.014.660112
TCGAGGG43500.014.4322184
CTCGAGG44800.013.4108523
AGCCGGC4400.012.7852872
TCCGGCG112450.012.6468043
CCGGCGC129300.012.1471674
Produced by FastQC (version 0.11.2)