FastQCFastQC Report
Wed 29 Oct 2014
uncut_3_CTGAAGCT-CCTATCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_3_CTGAAGCT-CCTATCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11544786
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6715815.817180153880722No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379430.32865918865884564No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225690.19549084755663726No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167580.14515643685383167No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164950.14287835218426742No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163770.141856245754577No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162650.14088611083826066No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT161450.13984668057077887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA65600.019.8368571
TGCGCGC8400.019.823193
GATCCGG207650.018.9413011
TGGGCGC5250.018.00163
TATCTCG43750.017.8473012
ATATCTC45000.017.6107961
TGGCGCG7850.017.4856323
ATCCGGC249200.016.036742
ATCTCGA92350.015.6755541
CTGCGCG7800.015.5783092
TATGGAT86100.015.4978942
ATAAAAA70700.014.7361052
TCTCGAG103700.014.6035162
TCGAGGG108200.014.3699424
AATAAAA73400.013.7413931
TCCGGCG295950.013.6555483
CCGGCGC329000.013.179234
CTCGAGG115100.013.0398213
CGGCGCG343600.012.6388745
TGGATCC114050.012.3701934
Produced by FastQC (version 0.11.2)