FastQCFastQC Report
Wed 29 Oct 2014
uncut_2_CTGAAGCT-ATAGAGGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_2_CTGAAGCT-ATAGAGGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4881892
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59114612.108952840415151No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148980.30516856989052604No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115680.23695731081310278No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103530.21206941898755646No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94140.19283507295941818No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71850.14717654548687273No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT71410.146275255577141No Hit
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62270.12755300608862302No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61720.12642639370145836No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAA60050.1230055888167948No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54380.11139123929820652No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT51470.10543043557702629No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48990.10035043790399295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA29600.024.9477061
ATATCTC18800.023.4718191
TGGGCGC2050.023.053453
ATAAAAA35100.021.2222482
TATCTCG21300.020.8140752
AATAAAA36200.020.212651
GATCCGG108300.019.6242031
TATGGAT38400.019.3398552
ATCTCGA39950.016.3429221
ATCCGGC134700.015.8551052
TCTCGAG44150.015.4957772
CGAGGGC78400.014.6741621
GTCGCGC1859.609539E-814.5975512
TGGATCC52000.014.4112784
GAGCCGG3800.014.2192321
TTCGGCG4000.014.0653512
ACCCGGC2102.9885996E-813.9313942
ACCGGCG7500.013.8027963
TCCGGCG160150.013.4619843
TCGAGGG52600.013.3484374
Produced by FastQC (version 0.11.2)